AMPK Beta 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AMPK Beta 1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 10308-1-AP

Synonyme

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 10308-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10308-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10308-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10308-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10308-1-AP

  IP result of anti-AMPK Beta 1 (IP:10308-1-AP, 4ug; Detection:10308-1-AP 1:5000) with mouse liver tissue lysate 1960 ug.

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  IHC staining of human lung cancer using 10308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10308-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMPK Beta 1 antibody (10308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A431-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

10308-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMPK Beta 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMPK Beta 1 fusion protein Ag0301
• Vollständiger Name: protein kinase, AMP-activated, beta 1 non-catalytic subunit
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC001007
• Gene symbol: PRKAB1
• Gene ID (NCBI): 5564
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).

Publikationen

Species	Application	Title
human	WB,IP	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
mouse	WB	Acta Pharm Sin B; Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy; Authors - Chang Xiao
mouse	WB,IF	Theranostics; Therapeutic inhibition of miR-802 protects against obesity through AMPK-mediated regulation of hepatic lipid metabolism.; Authors - Yangyue Ni
human,mouse	WB	J Ethnopharmacol; Mulberry leaves attenuate D-galactose-induced aging in vivo and in vitro; Authors - Yan Zhu
human	WB	Hum Cell; RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1; Authors - Jie Huang
rat	WB	Dig Dis Sci; Heme Oxygenase-1-Modified BMMSCs Activate AMPK-Nrf2-FTH1 to Reduce Severe Steatotic Liver Ischemia-Reperfusion Injury; Authors - Xiaorong Tian