PRG2 Polyklonaler Antikörper

PRG2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10766-1-AP

Synonyme

BMPG, Bone marrow proteoglycan, EMBP, MBP, MBP1, PRG2, Proteoglycan 2

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10766-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10766-1-AP (PRG2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10766-1-AP bindet in IF, IHC, ELISA PRG2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRG2 fusion protein Ag1150
• Vollständiger Name: proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein)
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC005929
• Gene symbol: PRG2
• Gene ID (NCBI): 5553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRG2, also known as BMPG, has 222 amino acid residues with a 16 N-terminal signal sequence, so the mature form has 206 amino acid residues. High levels of PRG2 are present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein can be cleaved into eosinophil granule major basic protein (MBP), which is the major constituent of the crystalline core of the eosinophil granule. MBP is highly basic and toxic to both mammalian cells and parasites, and it functions in the defense against invading parasites (PMID: 21270431).

Publikationen

Species	Application	Title
human	IF	Allergy; Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity; Authors - Jonathan G Medernach
human	IHC	Exp Eye Res; Role of TGF-β in tissue eosinophilia associated with vernal keratoconjunctivitis.; Authors - Ohtomo Kaori K