PREB Polyklonaler Antikörper

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• KD/KO Validated

PREB Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10146-2-AP

Synonyme

SEC12, Prolactin regulatory element-binding protein, Guanine nucleotide-exchange factor SEC12

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 10146-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 cells using 10146-2-AP

  WB result of anti-PREB (10146-2-AP, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.

• 

  WB analysis of HT-1080 using 10146-2-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10146-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10146-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10146-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 10146-2-AP

  IP result of anti-PREB (IP:10146-2-AP, 4ug; Detection:10146-2-AP 1:500) with SKOV-3 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10146-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PREB antibody (10146-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, 3T3-L1-Zellen, HepG2-Zellen, HT-1080.Zellen
• Erfolgreiche IP: SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10146-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PREB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PREB fusion protein Ag0202
• Vollständiger Name: prolactin regulatory element binding
• Berechnetes Molekulargewicht: 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC002765
• Gene symbol: PREB
• Gene ID (NCBI): 10113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

Publikationen

Species	Application	Title
mouse,human	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
	IP,WB,IF	Int J Biol Macromol; Cellular OCIAD2 protein is a proviral factor for hepatitis C virus replication.; Authors - Zibing Yang
human	WB,CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun ; KD Validated
human	WB	Biochem Biophys Res Commun; Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.; Authors - Yuri Urai
mouse	WB	Biochim Biophys Acta; Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis.; Authors - Joo-Man Park
human	WB	Virol J; PREB inhibits the replication of prototype foamy virus by affecting its transcription; Authors - Junshi Zhang ; KD Validated