PRDX3 Polyklonaler Antikörper

PRDX3 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 55087-1-AP

Synonyme

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, Peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

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Galerie der Validierungsdaten

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  WB analysis using 55087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55087-1-AP (PRDX3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55087-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55087-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55087-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55087-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55087-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55087-1-AP (Peroxiredoxin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55087-1-AP (PRDX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 55087-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55087-1-AP (Peroxiredoxin 3 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

55087-1-AP bindet in WB, IHC, ELISA PRDX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: peroxiredoxin 3
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 26-28 kDa
• GenBank-Zugangsnummer: NM_006793
• Gene symbol: PRDX3
• Gene ID (NCBI): 10935
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells(PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. This antibody is specific to PRDX3.

Publikationen

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
rat	WB	Br J Pharmacol; Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.; Authors - Bo Wei
human	WB	J Cell Biochem; Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.; Authors - Sulpizio Marilisa M
rat	WB	Cardiovasc Toxicol; Nrf1 Knock-Down in the Hypothalamic Paraventricular Nucleus Alleviates Hypertension Through Intervention of Superoxide Production-Removal Balance and Mitochondrial Function.; Authors - Ying Li