PRDM2 Polyklonaler Antikörper

PRDM2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 27718-1-AP

Synonyme

GATA 3 binding protein G3B, HUMHOXY1, KMT8, Lysine N methyltransferase 8, MTB ZF, MTE binding protein, PR domain containing protein 2, PRDM2, RIZ, RIZ1, RIZ2, Zinc finger protein RIZ

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  WB analysis of HeLa using 27718-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27718-1-AP (PRDM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

27718-1-AP bindet in WB, ELISA PRDM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PRDM2 fusion protein Ag26769
• Vollständiger Name: PR domain containing 2, with ZNF domain
• Berechnetes Molekulargewicht: 189 kDa
• Beobachtetes Molekulargewicht: 250-280 kDa
• GenBank-Zugangsnummer: NM_001007257
• Gene symbol: PRDM2
• Gene ID (NCBI): 7799
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PRDM2, also named as KMT8, RIZ, is a 1718 amino acid protein, which belongs to the class V-like SAM-binding methyltransferase superfamily. PRDM2 localizes in the nucleus and is highly expressed in retinoblastoma cell lines and in brain tumors and is also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3. PRDM2 as a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. The calculated molecular weight of PRDM2 is about 189 kDa. PRDM2 exists some isoforms with 250 kDa and 280 kDa. (PMID: 26040698)

Publikationen

Species	Application	Title
human	WB	Nat Commun; Combinatorial ubiquitin code degrades deubiquitylation-protected substrates; Authors - Mai Morita