PPP4C Polyklonaler Antikörper

PPP4C Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 10262-1-AP

Synonyme

PPP4, PPH3, PP4C, PP4, PP X

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10262-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10262-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10262-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10262-1-AP (PPP4C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10262-1-AP

  IP result of anti-PPP4C (IP:10262-1-AP, 4ug; Detection:10262-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IF Staining of HEK-293 using 10262-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 10262-1-AP (PPP4C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Produktinformation

10262-1-AP bindet in WB, IF/ICC, IP, ELISA PPP4C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP4C fusion protein Ag0356
• Vollständiger Name: protein phosphatase 4 (formerly X), catalytic subunit
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001416
• Gene symbol: PPP4C
• Gene ID (NCBI): 5531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

Publikationen

Species	Application	Title
	IP	Genes Dev; Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity.; Authors - Sabrina Klemz
	WB,IF,IP	Cell Rep; Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression; Authors - Marisa Connell ; KD Validated
mouse		Front Cardiovasc Med; Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.; Authors - Yue Zheng
human	WB	FEBS Lett; Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.; Authors - Chen Lu L
human	WB,IP	Cell Death Differ; AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner; Authors - Weimin Xu