PPP4C Polyklonaler Antikörper

PPP4C Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10262-1-AP

Synonyme

PPP4, PPH3, PP4C, PP4, PP X



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, Jurkat-Zellen, Mausnierengewebe
Erfolgreiche IPMausnierengewebe
Erfolgreiche Detektion in IF/ICCHEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10262-1-AP bindet in WB, IF/ICC, IP, ELISA PPP4C und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPP4C fusion protein Ag0356
Vollständiger Name protein phosphatase 4 (formerly X), catalytic subunit
Berechnetes Molekulargewicht 35 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC001416
Gene symbol PPP4C
Gene ID (NCBI) 5531
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Serine/threonine protein phosphatase X (PPX; also called protein phosphatase 4 (PP4)) are specifically associated with nuclear factor-kappa B (NF-kappa B) p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Overexpression of PPX stimulated the DNA-binding activity of c-Rel and activated NF-kappa B-mediated transcription. Although the mechanism by which PPX activates Rel/NF-kappa B-mediated transcription is unclear, PPX appeared to act on Rel/NF-kappa B proteins directly through augmentation of c-Rel activity. It is also possible that PPX may dephosphorylate and subsequently activate other c-Rel-associated transcription factors or other kinases regulating I kappaB (e.g. I kappa B kinases or MEKK1). These data suggest that PPX is an activator, but not an inhibitor, of c-Rel/NF-kappa B, which is in contrast to other protein phosphatases.

Protokolle

Produktspezifische Protokolle
WB protocol for PPP4C antibody 10262-1-APProtokoll herunterladen
IF protocol for PPP4C antibody 10262-1-APProtokoll herunterladen
IP protocol for PPP4C antibody 10262-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IP

Genes Dev

Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity.

Authors - Sabrina Klemz
WB,IF,IP

Cell Rep

Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

Authors - Marisa Connell
  • KD Validated
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
humanWB

FEBS Lett

Protein phosphatase 4 negatively regulates LPS cascade by inhibiting ubiquitination of TRAF6.

Authors - Chen Lu L
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu