PPP3R2 Polyklonaler Antikörper

PPP3R2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14005-1-AP

Synonyme

Calcineurin B like protein, Calcineurin BII, Calcineurin subunit B type 2, CBLP, CNBII, PPP3R2, PPP3RL

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 14005-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14005-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 14005-1-AP

  IP result of anti-PPP3R2 (IP:14005-1-AP, 4ug; Detection:14005-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human testis using 14005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 14005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 14005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 14005-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

14005-1-AP bindet in WB, IP, IF, IHC, ELISA PPP3R2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP3R2 fusion protein Ag5097
• Vollständiger Name: protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC030595
• Gene symbol: PPP3R2
• Gene ID (NCBI): 5535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Proc Natl Acad Sci U S A; SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.; Authors - Haruhiko Miyata
mouse	IHC	Cell Death Dis; miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.; Authors - Xudong Zhang
mouse	IF, IHC, WB	FASEB J; Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.; Authors - Souvik Dey
mouse	WB	Eur J Pharmacol; Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.; Authors - Jiali Zheng