PPP3R1 Polyklonaler Antikörper

PPP3R1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 13210-1-AP

Synonyme

Calcineurin subunit B type 1, CALNB1, CNA2, CNB, CNB1, PPP3R1

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 13210-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13210-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 13210-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13210-1-AP (PPP3R1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 13210-1-AP

  IP result of anti-PPP3R1 (IP:13210-1-AP, 3ug; Detection:13210-1-AP 1:300) with HeLa cells lysate 5000ug.

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  IHC staining of human gliomas tissue using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13210-1-AP( PPP3R1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas tissue using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13210-1-AP( PPP3R1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 13210-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13210-1-AP (PPP3R1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, humanes Magengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

13210-1-AP bindet in WB, IP, IF, IHC, ELISA PPP3R1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP3R1 fusion protein Ag3992
• Vollständiger Name: protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
• Berechnetes Molekulargewicht: 170 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC027913
• Gene symbol: PPP3R1
• Gene ID (NCBI): 5534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.

Publikationen

Species	Application	Title
human	WB	Acta Biomater; Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.; Authors - Hong Cao
mouse	WB	Proc Natl Acad Sci U S A; Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.; Authors - Xiju He
human	WB	Biochem Biophys Res Commun; The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages.; Authors - Su Zhenyi Z
chicken	WB	Biol Trace Elem Res; Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.; Authors - Ke Xiao
mouse	WB	Autophagy; Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin; Authors - Zhiyuan Fang
human	IF	Autophagy; SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis; Authors - Qin Xia