PPP2R2B/A/C/D Polyklonaler Antikörper

PPP2R2B/A/C/D Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 13123-1-AP

Synonyme

PPP2R2B, PP2A subunit B isoform beta, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA

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Galerie der Validierungsdaten

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  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13123-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13123-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13123-1-AP

  IP result of anti-PPP2R2B/A/C/D (IP:13123-1-AP, 4ug; Detection:13123-1-AP 1:500) with SH-SY5Y cells lysate 1200ug.

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  IHC staining of mouse brain using 13123-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13123-1-AP (PPP2R2B/A/C/D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13123-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2B/A/C/D antibody (13123-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

13123-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PPP2R2B/A/C/D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R2B/A/C/D fusion protein Ag3860
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Berechnetes Molekulargewicht: 443 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC031790
• Gene symbol: PPP2R2B
• Gene ID (NCBI): 5521
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The PPP2R2B gene encodes a deduced 443-amino acid protein of approximately 52 kDa,which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody has cross-reactivity to PPP2R2A, PPP2R2C and PPP2R2D.

Publikationen

Species	Application	Title
human	WB,CoIP	Mol Ther Nucleic Acids; miR21 modulates the Hippo signaling pathway via interference with PP2A Bβ to inhibit trophoblast invasion and cause preeclampsia; Authors - Mingyu Hu
human	WB,IHC,IF	Cancer Cell Int; PPP2R2B downregulation is associated with immune evasion and predicts poor clinical outcomes in triple-negative breast cancer.; Authors - Zheng Li