PPP2R1B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPP2R1B Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12621-1-AP

Synonyme

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PR65B, PP2A subunit A isoform R1-beta, PP2A subunit A isoform R1 beta, PP2A subunit A isoform PR65-beta

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Galerie der Validierungsdaten

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  WB analysis using 12621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12621-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12621-1-AP (PPP2R1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12621-1-AP (PPP2R1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Caco-2 using 12621-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using PPP2R1B antibody (12621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, HeLa-Zellen, humanes Nierengewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12621-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA PPP2R1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R1B fusion protein Ag3307
• Vollständiger Name: protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform
• Berechnetes Molekulargewicht: 667 aa, 74 kDa
• Beobachtetes Molekulargewicht: 60-74 kDa
• GenBank-Zugangsnummer: BC027596
• Gene symbol: PPP2R1B
• Gene ID (NCBI): 5519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Cell Dev Biol; BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.; Authors - Baoyu Chen
mouse	WB	EMBO Rep; CIP2A deficiency promotes depression-like behaviors in mice through inhibition of dendritic arborization; Authors - Wen-Ting Hu
human,mouse	WB,IHC,CoIP	Cancer Cell Int; PPP2R1B abolishes colorectal cancer liver metastasis and sensitizes Oxaliplatin by inhibiting MAPK/ERK signaling pathway; Authors - Wei Liu ; KD Validated