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PPP2CA Monoklonaler Antikörper

PPP2CA Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A6H5

Kat-Nr. : CL488-67809

Synonyme

PP2A alpha, PP2Ac, PP2CA, PPP2CA, Replication protein C, RP C

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated PPP2CA Monoclonal an (CL488-67809)

    FC experiment of MCF-7 using CL488-67809

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PPP2CA (CL488-67809, Clone:3A6H5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-67809 bindet in FC (Intra) PPP2CA und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PPP2CA fusion protein Ag4294
Vollständiger Name protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Berechnetes Molekulargewicht 309 aa, 36 kDa
Beobachtetes Molekulargewicht33 kDa
GenBank-ZugangsnummerBC031696
Gene symbol PPP2CA
Gene ID (NCBI) 5515
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

Protokolle

Produktspezifische Protokolle
FC protocol for CL Plus 488 PPP2CA antibody CL488-67809Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen