PPP2CA Polyklonaler Antikörper

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• KD/KO Validated

PPP2CA Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13482-1-PBS

Synonyme

EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac, PP2CA

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  WB analysis of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  WB analysis using 13482-1-AP (same clone as 13482-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 13482-1-AP (same clone as 13482-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 13482-1-AP (same clone as 13482-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

  IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 13482-1-AP (same clone as 13482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 13482-1-AP (same clone as 13482-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13482-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA PPP2CA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2CA fusion protein Ag4294
• Vollständiger Name: protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
• Berechnetes Molekulargewicht: 309 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC031696
• Gene symbol: PPP2CA
• Gene ID (NCBI): 5515
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.