PPP1R2P9 Polyklonaler Antikörper

PPP1R2P9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11969-1-AP

Synonyme

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Galerie der Validierungsdaten

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  WB analysis of human testis using 11969-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11969-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11969-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11969-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11969-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 11969-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 11969-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11969-1-AP (PPP1R2P9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11969-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPP1R2P9 antibody 11969-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11969-1-AP bindet in WB, IHC, IF/ICC, ELISA PPP1R2P9 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP1R2P9 fusion protein Ag2591
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 2 pseudogene 9
• Berechnetes Molekulargewicht: 202 aa, 23 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC056673
• Gene symbol: PPP1R2P9
• Gene ID (NCBI): 80316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.