CPI17 Polyklonaler Antikörper

CPI17 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11658-1-AP

Synonyme

PPP1R14A, Protein kinase C-potentiated inhibitor protein of 17 kDa, PPP1INL, CPI-17, CPI 17

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11658-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11658-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11658-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11658-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 11658-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11658-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11658-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11658-1-AP (CPI17 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 11658-1-AP

  Immunofluorescent analysis of PC-3 cells using 11658-1-AP (CPI17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Kolongewebe, Jurkat-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11658-1-AP bindet in WB, IF/ICC, ELISA CPI17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPI17 fusion protein Ag2237
• Vollständiger Name: protein phosphatase 1, regulatory (inhibitor) subunit 14A
• Berechnetes Molekulargewicht: 147 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC021089
• Gene symbol: CPI17
• Gene ID (NCBI): 94274
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1R14A, also named as CPI17, belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. CPI17 has two isoforms:CPI-17alpha (17 kDa) and CPI-17beta (13 kDa).