PPM1A Polyklonaler Antikörper

PPM1A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12961-1-AP

Synonyme

PP2C ALPHA, PP2CA, PPM1A, PPPM1A, Protein phosphatase 1A, Protein phosphatase IA

---

Galerie der Validierungsdaten

• 

  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12961-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12961-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12961-1-AP (PPM1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 12961-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 12961-1-AP (PPM1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, humanes Lebergewebe, K-562-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

12961-1-AP bindet in WB, IF, IHC, ELISA, Cell treatment PPM1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPM1A fusion protein Ag3645
• Vollständiger Name: protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• Berechnetes Molekulargewicht: 382 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC026691
• Gene symbol: PPM1A
• Gene ID (NCBI): 5494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPM1A(Protein phosphatase 1A) is also named as PPPM1A and belongs to the PP2C family. It is a serine/threonine protein phosphatase that is essential for regulating cellular stress responses in eukaryotes. PPM1A plays an important role in tumor suppression and that it negatively controls many signaling pathways which are critical for trophoblast invasion.(PMID:19404668). This protein distributes in both the cytoplasm and nucleus of mammalian cells(PMID:9003755). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	Cell treatment	Cell Rep; Blocking AMPK β1 myristoylation enhances AMPK activity and protects mice from high-fat diet-induced obesity and hepatic steatosis; Authors - Katyayanee Neopane
rat	IF	iScience; Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration; Authors - Haibin Tian
mouse	WB	Biol Pharm Bull; Sorghum (Sorghum bicolor) Extract-Induced Adipogenesis Is Independent of PPARγ Ser273 Phosphorylation in 3T3-L1 Adipocytes.; Authors - Aozora Nagaoka