PPIG Polyklonaler Antikörper

PPIG Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12985-1-AP

Synonyme

Cyclophilin G, Clk-associating RS-cyclophilin, CASP10, CARS-Cyp, CARS-cyclophilin

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Galerie der Validierungsdaten

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  WB analysis of human liver using 12985-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 12985-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12985-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12985-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 12985-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12985-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12985-1-AP (PPIG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 12985-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12985-1-AP (PPIG antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12985-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12985-1-AP (PPIG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12985-1-AP

  Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 12985-1-AP

  Immunofluorescent analysis of Hela cells, using PPIG antibody 12985-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, Jurkat-Zellen, NIH/3T3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

12985-1-AP bindet in WB, IHC, IF/ICC, ELISA PPIG und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPIG fusion protein Ag3622
• Vollständiger Name: peptidylprolyl isomerase G (cyclophilin G)
• Berechnetes Molekulargewicht: 739 aa, 87 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC001555
• Gene symbol: PPIG
• Gene ID (NCBI): 9360
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Sci Adv; High-throughput tagging of endogenous loci for rapid characterization of protein function; Authors - Joonwon Kim