CXCL7/PPBP Polyklonaler Antikörper

CXCL7/PPBP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13313-1-AP

Synonyme

PPBP,NAP2, CXCL7, NAP-2, PPBP, PPBP/NAP2

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  WB analysis of mouse liver using 13313-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 13313-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13313-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13313-1-AP (PPBP,NAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse placenta using 13313-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13313-1-AP (CXCL7/PPBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 13313-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13313-1-AP (CXCL7/PPBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Lebergewebe, humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13313-1-AP bindet in WB, IHC, ELISA CXCL7/PPBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL7/PPBP fusion protein Ag4147
• Vollständiger Name: pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• Berechnetes Molekulargewicht: 128 aa, 14 kDa
• Beobachtetes Molekulargewicht: 8-14 kDa
• GenBank-Zugangsnummer: BC028217
• Gene symbol: PPBP
• Gene ID (NCBI): 5473
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPBP, also named as CXCL7, NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi