PPAT Polyklonaler Antikörper

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• KD/KO Validated

PPAT Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 15401-1-AP

Synonyme

Amidophosphoribosyltransferase, ATASE, GPAT, PPAT, PRAT

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 15401-1-AP

  WB result of PPAT antibody (15401-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAT transfected HEK-293 cells.

• 

  WB analysis using 15401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15401-1-AP (PPAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15401-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15401-1-AP (PPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15401-1-AP

  IP Result of anti-PPAT (IP:15401-1-AP, 4ug; Detection:15401-1-AP 1:500) with HepG2 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 19 publications below

Produktinformation

15401-1-AP bindet in WB, IP, ELISA PPAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPAT fusion protein Ag7618
• Vollständiger Name: phosphoribosyl pyrophosphate amidotransferase
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC004200
• Gene symbol: PPAT
• Gene ID (NCBI): 5471
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB	Nat Commun; A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.; Authors - Manabu Kodama
human	WB	Nat Commun; Menin enhances c-Myc-mediated transcription to promote cancer progression.; Authors - Gongwei Wu
human	WB	EMBO J; UHMK1 promotes gastric cancer progression through reprogramming nucleotide metabolism.; Authors - Xing Feng ; KD Validated

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