PPAN Polyklonaler Antikörper

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• KD/KO Validated

PPAN Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11006-1-AP

Synonyme

BXDC3, Peter Pan homolog, peter pan homolog (Drosophila), PPAN, SSF, Ssf 1, SSF1, SSF2, Suppressor of SWI4 1 homolog

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Galerie der Validierungsdaten

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  WB analysis using 11006-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11006-1-AP (PPAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11006-1-AP

  WB result of PPAN antibody (11006-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAN transfected HEK-293 cells.

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  IHC staining of human colon using 11006-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11006-1-AP (PPAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mausherzgewebe, Rattenherzgewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Produktinformation

11006-1-AP bindet in WB, IP, IF, IHC, ELISA PPAN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPAN fusion protein Ag1419
• Vollständiger Name: peter pan homolog (Drosophila)
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa
• GenBank-Zugangsnummer: BC009833
• Gene symbol: PPAN
• Gene ID (NCBI): 56342
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.

Publikationen

Species	Application	Title
mouse	IP	EMBO J; The long non-coding RNA Paupar promotes KAP1-dependent chromatin changes and regulates olfactory bulb neurogenesis.; Authors - Ioanna Pavlaki
human	WB,IF,IP	Development; Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis.; Authors - Bugner Verena V
human	WB	Cancers (Basel); Nucleolar Stress Functions Upstream to Stimulate Expression of Autophagy Regulators.; Authors - David P Dannheisig ; KD Validated
human	WB, IF	J Biol Chem; The Wnt target Peter Pan defines a novel p53-independent nucleolar stress response pathway.; Authors - Astrid S Pfister ; KD Validated
xenopus	WB,IHC	Biol Cell; Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development.; Authors - Tecza Aleksandra A
human	WB	Cells; Loss of Peter Pan (PPAN) Affects Mitochondrial Homeostasis and Autophagic Flux.; Authors - David P Dannheisig ; KD Validated