PPP2R4 Polyklonaler Antikörper

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• KD/KO Validated

PPP2R4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10321-1-AP

Synonyme

PP2A, PP2A, subunit B, PR53 isoform, PPP2R4, PR53, PTPA

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10321-1-AP

  WB result of PP2A antibody (10321-1-AP, 1:600) with si-control and si-PP2A transfected HEK293 cells.

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  WB analysis using 10321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10321-1-AP (PPP2R4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 10321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 10321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 10321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10321-1-AP (PPP2R4 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IP: See 1 publications below

Produktinformation

10321-1-AP bindet in WB, IP, IHC, ELISA PPP2R4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPP2R4 fusion protein Ag0392
• Vollständiger Name: protein phosphatase 2A activator, regulatory subunit 4
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC002545
• Gene symbol: PPP2R4
• Gene ID (NCBI): 5524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; KDM6B promotes PARthanatos via suppression of O6-methylguanine DNA methyltransferase repair and sustained checkpoint response.; Authors - Mingming Yang
human	WB	EBioMedicine; Cancer-associated Fibroblasts Promote Irradiated Cancer Cell Recovery Through Autophagy.; Authors - Yongbin Wang
human		J Cell Sci; The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.; Authors - Liping Chen
human	WB	Mol Cancer Ther; Peptide PDHPS1 inhibits ovarian cancer growth through disrupting YAP signaling.; Authors - Xinxing Pan
human	WB	Front Immunol; CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.; Authors - Xiaowen Bi
human	WB	Life Sci; PP2A alleviates oxidized LDL-induced endothelial dysfunction by regulating LOX-1/ROS/MAPK axis.; Authors - Kaicheng Xu