OCT2 Polyklonaler Antikörper

OCT2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10867-2-AP

Synonyme

Oct 2, OCT2, Octamer binding protein 2, OTF 2, OTF2, POU class 2 homeobox 2, POU2F2

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 10867-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 10867-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10867-2-AP (OCT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 10867-2-AP

  IP result of anti-OCT2 (IP:10867-2-AP, 4ug; Detection:10867-2-AP 1:300) with NIH/3T3 cells lysate 1200ug.

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  IHC staining of human lymphoma using 10867-2-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10867-2-AP (OCT2 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maus-Eierstockgewebe
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

10867-2-AP bindet in WB, IHC, IP, ChIP, ELISA OCT2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OCT2 fusion protein Ag0955
• Vollständiger Name: POU class 2 homeobox 2
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC006101
• Gene symbol: POU2F2
• Gene ID (NCBI): 5452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POU2F2, also named as OCT2, is a 479 amino acid protein, which belongs to the POU transcription factor family. The Pit-Oct-Unc (POU) family of transcription factors includes many proteins that have been grouped in seven distinct classes according to function and expression containing proteins such as the neuronally expressed Pit proteins in class I, the ubiquitously expressed Oct-1 and tissue-specific Oct-2 in the second category, the well-known pluripotency marker Oct-4 which belongs to class V. Oct-1 (POU2f1) is a ubiquitous factor present in a wide range of cell types while Oct-2 (POU2f2) expression has been found confined to B lymphocytes and neuronal cells. Oct-2 has been implicated in B cell development and regulation of B lymphocyte-specific genes. POU2F2 exists various isoforms with MV 43, 50, and 62 kDa.

Publikationen

Species	Application	Title
human	WB,ChIP	Mol Cell; IκBζ is a dual-use coactivator of NF-κB and POU transcription factors; Authors - Aktan Alpsoy