POLR2C Polyklonaler Antikörper

POLR2C Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13428-1-AP

Synonyme

hRPB33, hsRPB3, POLR2C, RNA polymerase II subunit 3, RNA polymerase II subunit B3, RPB3, RPB31, RPB33

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13428-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13428-1-AP (POLR2C Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13428-1-AP

  IP result of anti-POLR2C (IP:13428-1-AP, 4ug; Detection:13428-1-AP 1:500) with HeLa cells lysate 2000ug.

• 

  IHC staining of human cervical cancer using 13428-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13428-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13428-1-AP (POLR2C antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

13428-1-AP bindet in WB, IP, IHC, ELISA POLR2C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2C fusion protein Ag4228
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide C, 33kDa
• Berechnetes Molekulargewicht: 275 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC028157
• Gene symbol: POLR2C
• Gene ID (NCBI): 5432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	Biomed Microdevices; Incorporation of VSV-G produces fusogenic plasma membrane vesicles capable of efficient transfer of bioactive macromolecules and mitochondria.; Authors - Hao-Peng Lin
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang