Pancreatic Lipase Polyklonaler Antikörper

Pancreatic Lipase Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11209-1-AP

Synonyme

Lipase, Pancreatic Lipase, PL, PNLIP, PTL

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Galerie der Validierungsdaten

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  WB analysis of BxPC-3 using 11209-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11209-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 11209-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 11209-1-AP (Pancreatic Lipase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

• 

  IHC staining of human pancreas using 11209-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 11209-1-AP (Pancreatic Lipase antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, humanes Pankreasgewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maus-Pankreasgewebe, humanes Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

11209-1-AP bindet in WB, IHC, ELISA Pancreatic Lipase und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Pancreatic Lipase fusion protein Ag1681
• Vollständiger Name: pancreatic lipase
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC014309
• Gene symbol: PNLIP
• Gene ID (NCBI): 5406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The pancreatic triacylglycerol lipase precursor (PNLIP), a 56 kDa protein secreted by the pancreas that is essential for the hydrolysis and absorption of long-chain triglyceride fatty acids in the intestine is located ~1 Mb upstream from the chromosome 10 peak(PMID:17890784). It is also named as PL, PTL and belongs to the AB hydrolase superfamily. It may potentially be involved in the pathophysiology of traumatic brain injury and may be implicated in the proliferation of astrocytes and the recovery of neurological outcomes(PMID:19760487). The full length protein has a signal peptide with 16 amino acid and a glycosylation site.

Publikationen

Species	Application	Title
mouse	IHC	Int J Mol Sci; Blocking β₁/β₂-Adrenergic Signaling Reduces Dietary Fat Absorption by Suppressing Expression of Pancreatic Lipase in High Fat-Fed Mice.; Authors - Kyunghwa Baek
pig	WB	JPEN J Parenter Enteral Nutr; Early Enteral Administration of a Complex Lipid Emulsion Supplement Prevents Postnatal Deficits in Docosahexaenoic and Arachidonic Acids and Increases Tissue Accretion of Lipophilic Nutrients in Preterm Piglets.; Authors - Olajumoke Akinsulire
human	IHC	Am J Cancer Res; A human pan-cancer system analysis of heat shock protein family A member 5; Authors - Chao Deng