PMM2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PMM2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10666-1-AP

Synonyme

phosphomannomutase 2, EC:5.4.2.8

---

Galerie der Validierungsdaten

• 

  WB analysis of multi-tissue using 10666-1-AP

  WB results of 10666-1-AP (PMM2 antibody) on several lysates.

• 

  WB analysis of HepG2 using 10666-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10666-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10666-1-AP (PMM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 10666-1-AP

  IP result of anti-PMM2 (IP:10666-1-AP, 4ug; Detection:10666-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

• 

  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10666-1-AP (PMM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10666-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10666-1-AP (PMM2 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of HepG2 using 10666-1-AP

  Immunofluorescent analysis of HepG2 cells using 10666-1-AP (PMM2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, multi-tissue
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10666-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PMM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMM2 fusion protein Ag1031
• Vollständiger Name: phosphomannomutase 2
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC008310
• Gene symbol: PMM2
• Gene ID (NCBI): 5373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMM2(Phosphomannomutase 2) belongs to the eukaryotic PMM family. It is involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. PMM2 catalyzes the second step in the conversion of fructose-6P to GDP-mannose.

Publikationen

Species	Application	Title
human	WB	Thromb Haemost; N-Glycosylation Deficiency Reduces the Activation of Protein C and Disrupts Endothelial Barrier Integrity; Authors - Tiffany Pascreau ; KD Validated
mouse	WB	J Proteome Res; Changes in the striatal proteome of YAC128Q mice exhibit gene-environment interactions between mutant huntingtin and manganese.; Authors - Wegrzynowicz Michal M
human	WB	Mol Genet Metab; Complex metabolic disharmony in PMM2-CDG paves the way to new therapeutic approaches; Authors - Nastassja Himmelreich
human	WB	J Inherit Metab Dis; Lack of phosphomannomutase 2 affects Xenopus laevis morphogenesis and the non-canonical Wnt5a/Ror2 signalling.; Authors - Nastassja Himmelreich
human	WB	Dis Model Mech; Repurposing the aldose reductase inhibitor and diabetic neuropathy drug epalrestat for the congenital disorder of glycosylation PMM2-CDG.; Authors - Sangeetha Iyer
	WB	PLoS One; Heterodimerization of Two Pathological Mutants Enhances the Activity of Human Phosphomannomutase2.; Authors - Giuseppina Andreotti