PLOD1 Polyklonaler Antikörper

PLOD1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12475-1-AP

Synonyme

LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

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Galerie der Validierungsdaten

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  WB analysis of Fibroblasts cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:200) with Protein extracts from Human Fibroblasts.

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  WB analysis of Mouse Fibroblast cells using 12475-1-AP

  WB result of PLOD1 antibody (12475-1-AP, 1:250) with Osteoblasts/Fibroblasts/714 cell line Lysate.

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of Mouse skin papilloma using 12475-1-AP

  IHC result of PLOD1 antibody (12475-1-AP, 1:100) with mouse skin papilloma slides.

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  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 12475-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12475-1-AP (PLOD1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Fibroblastenzellen, Maus-Fibroblastenzellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, ; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12475-1-AP bindet in WB, IF, IHC, ELISA PLOD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLOD1 fusion protein Ag3103
• Vollständiger Name: procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
• Berechnetes Molekulargewicht: 727 aa, 84 kDa
• Beobachtetes Molekulargewicht: 85-88 kDa
• GenBank-Zugangsnummer: BC016657
• Gene symbol: PLOD1
• Gene ID (NCBI): 5351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PLOD1, also named as LLH, PLOD and LH1, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. PLOD1 catalyses the hydroxylation of lysine residues during the posttranslational modification of type I collagen, the major protein of bone.

Publikationen

Species	Application	Title
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB,IHC	Bone; Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.; Authors - Tomoaki Saito
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima
mouse	WB	J Pharmacol Exp Ther; Minoxidil Cannot Be Used To Target Lysyl Hydroxylases during Postnatal Mouse Lung Development: A Cautionary Note.; Authors - Tilman Pfeffer
Bumblebees	IF	Front Genet; Mating Stimulates the Immune Response and Sperm Storage-Related Genes Expression in Spermathecae of Bumblebee (Bombus terrestris) Queen.; Authors - Yueqin Guo