tPA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

tPA Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 10147-1-AP

Synonyme

PLAT, T PA, Reteplase, EC:3.4.21.68, AlteplaseReteplase

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Galerie der Validierungsdaten

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  WB analysis using 10147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10147-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 10147-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10147-1-AP (tPA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10147-1-AP

  IP result of anti-tPA (IP:10147-1-AP, 3ug; Detection:10147-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10147-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10147-1-AP (tPA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10147-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 10147-1-AP (tPA antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Pankreasgewebe, Rattennierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Produktinformation

10147-1-AP bindet in WB, IHC, IF-P, IP, ELISA tPA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: tPA fusion protein Ag0200
• Vollständiger Name: plasminogen activator, tissue
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 32-35 kDa, 65 kDa
• GenBank-Zugangsnummer: BC002795
• Gene symbol: tPA
• Gene ID (NCBI): 5327
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Redox Biol; FUNDC1-dependent mitophagy induced by tPA protects neurons against cerebral ischemia-reperfusion injury.; Authors - Ying Cai ; KO Validated
mouse	IF	J Neurosci; Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile x mice.; Authors - V Swaroop Achuta
human	WB	Aging (Albany NY); NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.; Authors - Pingping Ma
mouse	IHC	Glia; Lipoprotein receptor loss in forebrain radial glia results in neurological deficits and severe seizures.; Authors - Ewa E Bres
human	WB	Sci Rep; Development of a Biomimetic Chondroitin Sulfate-modified Hydrogel to Enhance the Metastasis of Tumor Cells.; Authors - Yang Liu
mouse	IF	Cell Cycle; tPA promotes the proliferation of lung fibroblasts and activates the Wnt/β-catenin signaling pathway in idiopathic pulmonary fibrosis.; Authors - Ling Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

nuo (Verified Customer) (08-22-2021)

There is a band at around 64 kD as expected, although there were other nonspecific bindings.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

tPA Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:10147-1-AP)