PLAA Polyklonaler Antikörper

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• KD/KO Validated

PLAA Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12529-1-AP

Synonyme

DOA1, PLA2P, PLAA, PLAP

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 12529-1-AP

  WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells.

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  WB analysis of K-562 using 12529-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12529-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12529-1-AP

  IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug.

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  IHC staining of human lung cancer using 12529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12529-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12529-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12529-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, MCF-7-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12529-1-AP bindet in WB, IP, IF, IHC, ELISA PLAA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PLAA fusion protein Ag3224
• Vollständiger Name: phospholipase A2-activating protein
• Berechnetes Molekulargewicht: 738 aa, 81 kDa
• Beobachtetes Molekulargewicht: 72-74 kDa
• GenBank-Zugangsnummer: BC032551
• Gene symbol: PLAA
• Gene ID (NCBI): 9373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.

Publikationen

Species	Application	Title
mouse	WB, IHC	Anesthesiology; miR-203 regulates nociceptive sensitization after incision by controlling phospholipase A2 activating protein expression.; Authors - Sun Yuan Y
human	WB,IF	Biochim Biophys Acta Mol Cell Res; Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization; Authors - Zhilong Zheng ; KD Validated
human	WB	Protein Cell; Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis; Authors - Xiaoguang Liu