PKC Zeta Polyklonaler Antikörper

PKC Zeta Polyklonal Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Kat-Nr. : CL488-26899

Synonyme

PKC ZETA, PKC2, PRKCZ, Protein kinase C zeta type, protein kinase C, zeta

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Galerie der Validierungsdaten

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  IF Staining of NIH/3T3 using CL488-26899

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 PKC Zeta antibody (CL488-26899) at dilution of 1:200.

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  IF Staining of HeLa using CL488-26899

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PKC Zeta antibody (CL488-26899) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: NIH/3T3-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-26899 bindet in IF PKC Zeta und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PKC Zeta fusion protein Ag25391
• Vollständiger Name: protein kinase C, zeta
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC014270
• Gene symbol: PRKCZ
• Gene ID (NCBI): 5590
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.