PKM2-specific Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PKM2-specific Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ICC, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

40

CloneNo.

1C11C7

Rezensionen (1)

Kat-Nr. : 60268-1-Ig

Synonyme

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 60268-1-Ig

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells.

• 

  WB analysis using 60268-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of human brain using 60268-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60268-1-Ig

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60268-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60268-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60268-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 60268-1-Ig

  Western blot analysis of PK-M2-specific in various tissues and cell lines using Proteintech antibody 60268-1-Ig at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60268-1-Ig

  IP Result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HeLa using 60268-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 60268-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

• 

  FC experiment of HepG2 using 60268-1-Ig

  1X10^6 HepG2 cells were stained with .2ug PKM2-specific antibody (60268-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 4T1-Zellen, C6-Zellen, HepG2-Zellen, humanes Hirngewebe, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe, ROS1728-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:30000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 34 publications below
• IHC: See 7 publications below
• IF: See 16 publications below
• IP: See 5 publications below
• CoIP: See 4 publications below

Produktinformation

60268-1-Ig bindet in WB, IP, IHC, IF, ICC, FC, CoIP, ELISA PKM2-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PKM pyruvate kinase, muscle
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_002654
• Gene symbol: PKM
• Gene ID (NCBI): 5315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Publikationen

Species	Application	Title
human	WB,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
rat	WB,IF,ICC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu ; KD Validated
human	WB	Autophagy; SQSTM1-dependent autophagic degradation of PKM2 inhibits the production of mature IL1B/IL-1β and contributes to LIPUS-mediated anti-inflammatory effect.; Authors - Bin Zhang
rat	WB	Acta Physiol (Oxf); Supra-coronary aortic banding improves right ventricular function in experimental pulmonary arterial hypertension in rats by increasing systolic right coronary artery perfusion.; Authors - Lian Tian
human	WB,IHC,IF	Mol Ther Nucleic Acids; Endoplasmic reticulum stress promotes sorafenib resistance via miR-188-5p/hnRNPA2B1-mediated upregulation of PKM2 in hepatocellular carcinoma; Authors - Bei Zhou ; KD Validated
mouse	WB	Int Immunopharmacol; Protein 4.1R regulates M1 macrophages polarization via glycolysis, alleviating sepsis-induced liver injury in mice; Authors - Si-Yao Sang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:60268-1-Ig)

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