PIP5K3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIP5K3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA, Dot Blot

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 13361-1-AP

Synonyme

CFD, FAB1, KIAA0981, PIKFYVE, PIP5K, PIP5K3, PIPkin III, Type III PIP kinase

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13361-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13361-1-AP (PIP5K3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13361-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13361-1-AP (PIP5K3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Mauslungengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

13361-1-AP bindet in WB, IHC, ELISA, Dot Blot PIP5K3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIP5K3 fusion protein Ag4177
• Vollständiger Name: phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
• Berechnetes Molekulargewicht: 451 aa, 50 kDa, 237 kDa
• Beobachtetes Molekulargewicht: 237 kDa
• GenBank-Zugangsnummer: BC032389
• Gene symbol: PIP5K3
• Gene ID (NCBI): 200576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIP5K3 (1-phosphatidylinositol 3-phosphate 5-kinase), also callled PIKFYVE, belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol (PMID: 15902656; 9858586). The content of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4 (PMID: 17556371). In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity (PubMed: 11714711; 17556371).PIP5K3 has four isoforms with molecular weights of 237 kDa, 50 kDa, 51 kDa and 62 kDa. This antibody primarily recognizes the 237 kDa isoform.

Publikationen

Species	Application	Title
		Mol Cell; TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation.; Authors - Katharina Fitzian ; KD Validated
human	WB	Elife; Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.; Authors - Sai Srinivas Panapakkam Giridharan
pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
human	IHC	Front Oncol; TRIM68, PIKFYVE, and DYNLL2: The Possible Novel Autophagy- and Immunity-Associated Gene Biomarkers for Osteosarcoma Prognosis.; Authors - Jie Jiang
		Eur J Pharmacol; Inhibition of c-Rel DNA binding is critical for the anti-inflammatory effects of novel PIKfyve inhibitor.; Authors - Masaomi Terajima
mouse	Dot Blot	BMC Immunol; miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.; Authors - Jingsong Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kincaid (Verified Customer) (05-21-2024)

This antibody works very well. I use it 1:200 - overnight incubation at 4°C (3% BSA in TBST). I have validated with H4 cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: H4 cell

PIP5K3 Antibody Western Blot validation (1:200 dilution) in H4 cell (Cat no:13361-1-AP)