PINX1 Polyklonaler Antikörper

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• KD/KO Validated

PINX1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12368-1-AP

Synonyme

Pin2-interacting protein X1, Pin2 interacting protein X1, PIN2 interacting protein 1, LPTS, LPTL

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Galerie der Validierungsdaten

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  WB analysis using 12368-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 12368-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 12368-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12368-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12368-1-AP (PINX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 12368-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12368-1-AP (PINX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen, C6-Zellen, HeLa-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 8 publications below

Produktinformation

12368-1-AP bindet in WB, IHC, IF/ICC, ELISA PINX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PINX1 fusion protein Ag3043
• Vollständiger Name: PIN2-interacting protein 1
• Berechnetes Molekulargewicht: 328 aa, 37 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC015479
• Gene symbol: PINX1
• Gene ID (NCBI): 54984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.

Publikationen

Species	Application	Title
human	WB	Oncogene; Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation.; Authors - Zhang B B ; KD Validated
human	IHC	Mol Cancer; The depletion of PinX1 involved in the tumorigenesis of non-small cell lung cancer promotes cell proliferation via p15/cyclin D1 pathway.; Authors - Xiao-Peng Tian
human	WB,IHC	Am J Cancer Res; PINX1 promotes malignant transformation of thyroid cancer through the activation of the AKT/MAPK/β-catenin signaling pathway.; Authors - JiHoon Kang ; KD Validated
human	WB,IHC	Mol Cancer; PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway.; Authors - Liu Jian-Ye JY
human	WB,IHC	Cancer Lett; PMID: 25045845; Authors - Xiao-Peng Tian
human	WB,IHC	Cancer Sci; Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma.; Authors - Cai Mu-Yan MY