PIN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIN1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

37

Rezensionen (1)

Kat-Nr. : 10495-1-AP

Synonyme

Pin 1, Peptidyl-prolyl cis-trans isomerase Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, EC:5.2.1.8

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10495-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10495-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10495-1-AP (PIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10495-1-AP

  IP result of anti-PIN1 (IP:10495-1-AP, 4ug; Detection:10495-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human renal cell carcinoma using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10495-1-AP (PIN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10495-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10495-1-AP (PIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of HEK-293 cells using 10495-1-AP (PIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 10495-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PIN1 antibody (10495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 11 publications below
• WB: See 34 publications below
• IHC: See 8 publications below
• IF: See 10 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10495-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PIN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIN1 fusion protein Ag0767
• Vollständiger Name: peptidylprolyl cis/trans isomerase, NIMA-interacting 1
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002899
• Gene symbol: PIN1
• Gene ID (NCBI): 5300
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Publikationen

Species	Application	Title
human	WB,IF,IP	Nat Commun; Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells; Authors - Qiuhong Zhu
human,mouse	WB	J Pineal Res; Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.; Authors - Dongmei Chen
human	WB,IF	J Hazard Mater; Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.; Authors - Fuli Zheng
human	WB	Theranostics; SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.; Authors - Ling Lin
human	WB	Nucleic Acids Res; Pin1 promotes GR transactivation by enhancing recruitment to target genes.; Authors - Poolman Toryn M TM ; KD Validated
mouse	WB, IHC, IF	Oxid Med Cell Longev; PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway.; Authors - Yanzhuo Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other