Piezo1 (extracellular domain) Polyklonaler Antikörper

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• KD/KO Validated

Piezo1 (extracellular domain) Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 28511-1-AP

Synonyme

FAM38A, KIAA0233, Mib, PIEZO1, PIEZO1 (extracellular domain)

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 28511-1-AP

  WB result of Piezo1 (extracellular domain) antibody (28511-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and Piezo1 (extracellular domain) knockout HeLa cells.

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  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28511-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28511-1-AP (Piezo1 (extracellular domain) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28511-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28511-1-AP (PIEZO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, hTERT-RPE1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

28511-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA Piezo1 (extracellular domain) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag29858
• Vollständiger Name: family with sequence similarity 38, member A
• Berechnetes Molekulargewicht: 286 kDa
• Beobachtetes Molekulargewicht: 280-300 kDa
• GenBank-Zugangsnummer: BC008073
• Gene symbol: FAM38A
• Gene ID (NCBI): 9780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells which influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as PIEZO1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Fam38A also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in gene encoding PIEZO1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1.

Publikationen

Species	Application	Title
mouse	WB,IF	Glia; Mechanosensitive channel Piezo1 is an essential regulator in cell cycle progression of optic nerve head astrocytes; Authors - Yue Wan ; KD Validated
human	WB,IF,IP	FASEB J; Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells.; Authors - Kathrin Diem ; KD Validated
human	WB	JCI Insight; Mechanosensitive Piezo1 channels mediate renal fibrosis.; Authors - Xiaoduo Zhao
human	WB,CoIP	Heliyon; The function of Piezo1 in hepatoblastoma metastasis and its potential transduction mechanism; Authors - Xiaoshuo Ye ; KD Validated
mouse	WB,IF	Neuron; Microglial Piezo1 senses Aβ fibril stiffness to restrict Alzheimer's disease; Authors - Jin Hu