• Überblick
  • Geprüfte Anwendungen
  • Produktinformation
  • Publikationen (11)
  • Protokolle
  • Rezensionen (1)
  • Featured Product
  • KD/KO Validated

PI4KA Polyklonaler Antikörper

PI4KA Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12411-1-AP

Synonyme

PIK4, PI4-kinase alpha, PI4K-alpha, Phosphatidylinositol 4-Kinase III alpha, Phosphatidylinositol 4-kinase alpha



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, humanes Hirngewebe, Maushirngewebe, MCF-7-Zellen, NIH-3T3 Zellen (RNAi), Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

12411-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PI4KA und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PI4KA fusion protein Ag3062
Vollständiger Name phosphatidylinositol 4-kinase, catalytic, alpha
Berechnetes Molekulargewicht 2044 aa, 231 kDa
Beobachtetes Molekulargewicht 231 kDa
GenBank-ZugangsnummerBC018120
Gene symbol PI4KA
Gene ID (NCBI) 5297
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PI4KA, also named as PIK4, PIK4CA, belongs to the PI3/PI4-kinase family and Type III PI4K subfamily. It acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate. PI4KA plays a role in the formation of membrane complexes where HCV replication takes place. (PMID:19605471).

Protokolle

Produktspezifische Protokolle
WB protocol for PI4KA antibody 12411-1-APProtokoll herunterladen
IHC protocol for PI4KA antibody 12411-1-APProtokoll herunterladen
IF protocol for PI4KA antibody 12411-1-APProtokoll herunterladen
IP protocol for PI4KA antibody 12411-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB

Nature

A phosphoinositide signalling pathway mediates rapid lysosomal repair

Authors - Jay Xiaojun Tan
  • KD Validated
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
humanWB,IF

Brain

Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with hypomyelinating leukodystrophy.

Authors - Edgard Verdura
humanIF

EMBO J

Defining the proximal interaction networks of Arf GTPases reveals a mechanism for the regulation of PLD1 and PI4KB.

Authors - Zhengming Wu
human,mouseWB,IHC,IF

Cell Rep

A Forward Genetic Screen Targeting the Endothelium Reveals a Regulatory Role for the Lipid Kinase Pi4ka in Myelo- and Erythropoiesis.

Authors - Safiyyah Ziyad
  • KD Validated
humanWB

Biomaterials

Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells.

Authors - Ming Gao
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Patricia (Verified Customer) (06-16-2024)

Works well with 30ug of cell lysate

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: immune cells

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