PHF20 Polyklonaler Antikörper

PHF20 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 22010-1-AP

Synonyme

C20orf104, GLEA2, Glioma expressed antigen 2, HCA58, Novel zinc finger protein, NZF, PHD finger protein 20, PHF20, Transcription factor TZP, TZP

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 22010-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22010-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22010-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22010-1-AP (PHF20 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 22010-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22010-1-AP (PHF20 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

22010-1-AP bindet in WB, IF PHF20 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHF20 fusion protein Ag15686
• Vollständiger Name: PHD finger protein 20
• Berechnetes Molekulargewicht: 1012 aa, 115 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC150178
• Gene symbol: PHF20
• Gene ID (NCBI): 51230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHD finger protein 20 (PHF20),also known as NZF, TZP, GLEA2 or HCA58, is a 1,012 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger, one C2H2-type zinc finger and one A/T hook DNA-binding domain.PHF20 contains a tudor domain, which facilitates binding to di-methylated histone H4 Lys20. PHF20 is expressed in a variety of tissues, including liver, heart, lung, pancreas, spleen, testis and placenta, PHF20 is thought to function as a transcription factor, possibly playing a role in the development of cancers, including glioblastoma, lung cancer, colon cancer and ovarian cancer. PHF20 exists as three alternatively spliced isoforms (150kd,130kd and 120kd) which are encoded by a gene that maps to human chromosome 20 (22072714).

Publikationen

Species	Application	Title
human	WB	Biochem Genet; Circular RNA circKIF2A Contributes to the Progression of Neuroblastoma Through Regulating PRPS1 Expression by Sponging miR-377-3p.; Authors - Quan Jin