Prohibitin 2 Polyklonaler Antikörper

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• KD/KO Validated

Prohibitin 2 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Electrochemical Immunosensor

Konjugation

Unkonjugiert

Publikationen

46

Kat-Nr. : 12295-1-AP

Synonyme

PHB2, Prohibitin-2, D-prohibitin, D prohibitin, B-cell receptor-associated protein BAP37

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Galerie der Validierungsdaten

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  WB analysis using 12295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12295-1-AP

  WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

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  IP experiment of HeLa using 12295-1-AP

  IP result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.

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  IP experiment of mouse brain using 12295-1-AP

  IP result of anti-Prohibitin 2 (IP:12295-1-AP, 4ug; Detection:12295-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 40 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

12295-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA, Electrochemical Immunosensor Prohibitin 2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Reis
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Prohibitin 2 fusion protein Ag2977
• Vollständiger Name: prohibitin 2
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC014766
• Gene symbol: Prohibitin 2
• Gene ID (NCBI): 11331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Publikationen

Species	Application	Title
human	WB	Mol Cell; Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria; Authors - Sabine Poerschke
human	WB	J Am Soc Nephrol; Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.; Authors - Sung-Gyu Cho
mouse	WB	Neuron; Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.; Authors - Kelly A Chamberlain
rat	IF	Nat Commun; Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.; Authors - Y Poitelon
mouse		Redox Biol; PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function; Authors - Mingjie Yang
human	IF	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu