PGK2 Polyklonaler Antikörper

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• KD/KO Validated

PGK2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13686-1-AP

Synonyme

PGKPS, PGKB, EC:2.7.2.3

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 13686-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 13686-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13686-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13686-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13686-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13686-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13686-1-AP (PGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 13686-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13686-1-AP (PGK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 13686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (13686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Hodengewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

13686-1-AP bindet in WB, IHC, IF-P, ELISA PGK2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PGK2 fusion protein Ag4553
• Vollständiger Name: phosphoglycerate kinase 2
• Berechnetes Molekulargewicht: 417 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC038843
• Gene symbol: PGK2
• Gene ID (NCBI): 5232
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

Publikationen

Species	Application	Title
mouse	WB	Chemosphere; Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.; Authors - Yongli Han
mouse	WB	Cell Physiol Biochem; Effect of Type I Diabetes on the Proteome of Mouse Oocytes.; Authors - Guangjian Jiang
sheep	WB	Theriogenology; Characterization of GALNTL5 gene sequence and expression in ovine testes and sperm.; Authors - Xiaolei Yao
mouse	WB	Nature; A male germ-cell-specific ribosome controls male fertility; Authors - Huiling Li
human	WB	Cell Res; Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate; Authors - Yu-Qing Wu ; KD Validated