Placental Growth Factor Polyklonaler Antikörper

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• KD/KO Validated

Placental Growth Factor Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 10642-1-AP

Synonyme

D12S1900, PGF, PGFL, PIGF, Placenta growth factor, placental growth factor, PLGF, PlGF 2, SHGC 10760

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Galerie der Validierungsdaten

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  WB analysis using 10642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10642-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10642-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human meningioma using 10642-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma using 10642-1-AP (Placental Growth Factor antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, Maus-Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Mammakarzinomgewebe, humanes Meningiom-Gewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• IHC: See 8 publications below
• IF: See 9 publications below

Produktinformation

10642-1-AP bindet in IHC, IF, ELISA Placental Growth Factor und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Placental Growth Factor fusion protein Ag0969
• Vollständiger Name: placental growth factor
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC007789
• Gene symbol: PGF
• Gene ID (NCBI): 5228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Placenta growth factor (PLGF) is a homodimeric glycoprotein, 46-50 kDa in size, belonging to the vascular endothelial growth factor (VEGF) sub-family. It plays an important role in pathological VEGF-driven angiogenesis. PLGF is expressed not only in placental cells but also in colon and mammary carcinomas. It acts as a primary inflammatory instigator of atherosclerotic plaque instability and thus may be useful as a risk-predicting biomarker in patients with acute coronary syndromes (ACS).

Publikationen

Species	Application	Title
rat	IF	Brain.; Global gene expression profiling of somatic motor neuron populations with different vulnerability identify molecules and pathways of degeneration and protection.; Authors - Hedlund E
mouse		Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song ; KD Validated
mouse	IHC,IF	Biomed Pharmacother; HIF-1α inhibitor YC-1 suppresses triple-negative breast cancer growth and angiogenesis by targeting PlGF/VEGFR1-induced macrophage polarization; Authors - Yan Li
human	IHC	Cancers (Basel); Occurrence of Fibrotic Tumor Vessels in Grade I Meningiomas Is Strongly Associated with Vessel Density, Expression of VEGF, PlGF, IGFBP-3 and Tumor Recurrence.; Authors - Katharina Hess
mouse	IF	Diabetes Obes Metab; Polarized macrophages promote gestational beta cell growth through extracellular signal-regulated kinase 5 signalling.; Authors - Yinan Jiang
mouse	IF	Int J Mol Sci; Alzheimer's Amyloid β Peptide Induces Angiogenesis in an Alzheimer's Disease Model Mouse through Placental Growth Factor and Angiopoietin 2 Expressions; Authors - Abdullah Md Sheikh