PFKFB2 Polyklonaler Antikörper

PFKFB2 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17838-1-AP

Synonyme

6PF 2 K/Fru 2,6 P2ase 2, PFK 2/FBPase 2, PFK/FBPase 2, PFKFB2

---

Galerie der Validierungsdaten

• 

  WB analysis of human heart using 17838-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17838-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17838-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 17838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17838-1-AP (PFKFB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17838-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17838-1-AP (PFKFB2 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

17838-1-AP bindet in WB, IHC,ELISA PFKFB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFKFB2 fusion protein Ag12181
• Vollständiger Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Berechnetes Molekulargewicht: 505 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 110 kDa
• GenBank-Zugangsnummer: BC075076
• Gene symbol: PFKFB2
• Gene ID (NCBI): 5208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family.The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway.; Authors - Shu-Jie Zhao
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	J Biol Chem; Type 2 Diabetes Dysregulates Glucose Metabolism in Cardiac Progenitor Cells.; Authors - Joshua K Salabei
human	WB	Int J Biochem Cell Biol; A transcriptional target of androgen receptor, miR-421 regulates proliferation and metabolism of prostate cancer cells.; Authors - Delong Meng
human	WB	J Transl Med; Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.; Authors - Telang Sucheta S