PFKFB2 Polyklonaler Antikörper

PFKFB2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17838-1-AP

Synonyme

Fructose-2,6-bisphosphatase, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2, 6-phosphofructo-2-kinase, 6PF-2-K/Fru-2,6-P2ase heart-type isozyme, 6PF-2-K/Fru-2,6-P2ase 2

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  WB analysis using 17838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse retina using 17838-1-AP

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 17838-1-AP (PFKFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Maus-Retina-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

17838-1-AP bindet in WB, ELISA PFKFB2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PFKFB2 fusion protein Ag12181
• Vollständiger Name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
• Berechnetes Molekulargewicht: 505 aa, 58 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC075076
• Gene symbol: PFKFB2
• Gene ID (NCBI): 5208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PFKFB2(6-Phosphofructo-2-kinase/fructose 2,6-bisphosphatase 2) is also named as PFK-2/FBPase-2, which is a bifunctional enzyme responsible for the synthesis and breakdown of Fru-2,6-P2 in the regulation of glycolysis(PMID:10095107). In the C-terminal section, it belongs to the phosphoglycerate mutase family. The enzyme is a dimer of identical subunits, each composed of 505 amino acids and containing three discrete domains(PMID: 2552438). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Cell Death Dis; SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway.; Authors - Shu-Jie Zhao
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong
mouse	WB	J Biol Chem; Type 2 Diabetes Dysregulates Glucose Metabolism in Cardiac Progenitor Cells.; Authors - Joshua K Salabei
human	WB	Int J Biochem Cell Biol; A transcriptional target of androgen receptor, miR-421 regulates proliferation and metabolism of prostate cancer cells.; Authors - Delong Meng
human	WB	J Transl Med; Small molecule inhibition of 6-phosphofructo-2-kinase suppresses t cell activation.; Authors - Telang Sucheta S