PEX1 Polyklonaler Antikörper

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• KD/KO Validated

PEX1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 13669-1-AP

Synonyme

PEX, Peroxisome biogenesis factor 1, Peroxisomal ATPase PEX1, Peroxin-1, Peroxin 1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 13669-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13669-1-AP (PEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 13669-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13669-1-AP (PEX1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 13669-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13669-1-AP

  Immunofluorescent analysis of HeLa cells using 13669-1-AP (PEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 13669-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 13669-1-AP (PEX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

13669-1-AP bindet in WB, IHC, IF/ICC, ELISA PEX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PEX1 fusion protein Ag4623
• Vollständiger Name: peroxisomal biogenesis factor 1
• Berechnetes Molekulargewicht: 1283 aa, 143 kDa
• Beobachtetes Molekulargewicht: 143 kDa
• GenBank-Zugangsnummer: BC035575
• Gene symbol: PEX1
• Gene ID (NCBI): 5189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	IF	Autophagy; Export-deficient monoubiquitinated PEX5 triggers peroxisome removal in SV40 large T antigen-transformed mouse embryonic fibroblasts.; Authors - Marcus Nordgren
human	WB,IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated
human	WB	Autophagy; AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.; Authors - Zhenhong Ni
mouse	WB	Cell Commun Signal; TFEB activation triggers pexophagy for functional adaptation during oxidative stress under calcium deficient-conditions; Authors - Laxman Manandhar
human	IHC	Eur J Hum Genet; Spectrum of PEX1 and PEX6 variants in Heimler syndrome.; Authors - Claire E L Smith
human	WB	Mol Ther Methods Clin Dev; AAV-mediated PEX1 gene augmentation improves visual function in the PEX1-Gly844Asp mouse model for mild Zellweger spectrum disorder.; Authors - Catherine Argyriou