PES1 Polyklonaler Antikörper

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• KD/KO Validated

PES1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13553-1-AP

Synonyme

PES

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Galerie der Validierungsdaten

• 

  WB analysis of COLO 320 using 13553-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13553-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 13553-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13553-1-AP (PES1 antibody) at dilution of 1:300.

• 

  IHC staining of human gliomas using 13553-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13553-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13553-1-AP (PES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 13553-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13553-1-AP (PES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, MCF-7-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13553-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PES1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PES1 fusion protein Ag4342
• Vollständiger Name: pescadillo homolog 1, containing BRCT domain (zebrafish)
• Berechnetes Molekulargewicht: 588 aa, 68 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC032489
• Gene symbol: PES1
• Gene ID (NCBI): 23481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Mol Med; Ketogenic diet ameliorates lipid dysregulation in type 2 diabetic mice by downregulating hepatic pescadillo 1.; Authors - Jielin Zhou
human	IHC,WB	Cancer Med; PES1 is a biomarker of head and neck squamous cell carcinoma and is associated with the tumor microenvironment; Authors - Yaodong He
human	WB,IHC,IP	J Exp Clin Cancer Res; SNHG17 promotes colorectal tumorigenesis and metastasis via regulating Trim23-PES1 axis and miR-339-5p-FOSL2-SNHG17 positive feedback loop.; Authors - Zehua Bian
human	WB,IHC	Life Sci; PES1 enhances proliferation and tumorigenesis in hepatocellular carcinoma via the PI3K/AKT pathway.; Authors - Jing Wang ; KD Validated
goat	WB	Int J Mol Sci; Integrative Proteomics and Transcriptomics Profiles of the Oviduct Reveal the Prolificacy-Related Candidate Biomarkers of Goats (Capra hircus) in Estrous Periods; Authors - Zhipeng Sun