Twinkle/PEO1 Polyklonaler Antikörper

Twinkle/PEO1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 13435-1-AP

Synonyme

ATXN8, C10orf2, IOSCA, PEO, PEO1, PEOA3, SANDO, SCA8, Twinkle, Twinkle protein, mitochondrial, TWINL

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 13435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13435-1-AP (Twinkle antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13435-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13435-1-AP (Twinkle antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 13435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13435-1-AP (Twinkle antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 13435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 13435-1-AP (Twinkle antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

13435-1-AP bindet in WB, IHC, ELISA Twinkle/PEO1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Twinkle/PEO1 fusion protein Ag4239
• Vollständiger Name: chromosome 10 open reading frame 2
• Berechnetes Molekulargewicht: 684 aa, 77 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC033762
• Gene symbol: PEO1
• Gene ID (NCBI): 56652
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Twinkle, also named as C10orf2, PEO1, is a 684 amino acid protein, which forms multimers in vitro, including hexamers. Twinkle colocalizes with mtDNA in mitochondrial nucleoids, a nucleoproteins complex consisting of a number of copies of proteins associated with mtDNA, probably involved in mtDNA maintenance and expression. Twinkle involves in mitochondrial DNA (mtDNA) metabolism and could function as an adenine nucleotide-dependent DNA helicase. Twinkle exists various isoform and molecular weight of isoform is 60 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis.; Authors - Vaibhav Sidarala
human		EMBO J; ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.; Authors - Li Shu
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li
mouse	WB	Sci Rep; PMID: 28397817; Authors - Xinbin Zhao
human	WB	Nucleic Acids Res; A deafness-associated mitochondrial DNA mutation caused pleiotropic effects on DNA replication and tRNA metabolism; Authors - Feilong Meng