PDZK1 Polyklonaler Antikörper

PDZK1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10507-2-AP

Synonyme

CAP70, CLAMP, NaPi Cap1, NHERF 3, NHERF3, PDZ domain containing 1, PDZD1, PDZK1

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 10507-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10507-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10507-2-AP (PDZK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10507-2-AP

  IP result of anti-PDZK1 (IP:10507-2-AP, 3ug; Detection:10507-2-AP 1:500) with HEK-293 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• CoIP: See 1 publications below

Produktinformation

10507-2-AP bindet in WB, IHC, IP, CoIP, ELISA PDZK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDZK1 fusion protein Ag0787
• Vollständiger Name: PDZ domain containing 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC006496
• Gene symbol: PDZK1
• Gene ID (NCBI): 5174
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Downregulation of vimentin expression increased drug resistance in ovarian cancer cells.; Authors - Yi Huo
human	IHC	Mol Med; PDZK1 Is a Novel Factor in Breast Cancer That Is Indirectly Regulated by Estrogen through IGF-1R and Promotes Estrogen-Mediated Growth.; Authors - Kim Hogyoung H
human	IHC	Mol Med; Correlation between PDZK1, Cdc37, Akt and breast cancer malignancy: the role of PDZK1 in cell growth through Akt stabilization by increasing and interacting with Cdc37.; Authors - Hogyoung Kim
human	WB,IHC	Cell Div; miR-101-3p-mediated role of PDZK1 in hepatocellular carcinoma progression and the underlying PI3K/Akt signaling mechanism; Authors - Huihui Gao ; KD Validated
human	WB,IHC,CoIP	Aging (Albany NY); Promoter hypomethylated PDZK1 acts as a tumorigenic gene in glioma by interacting with AKT1; Authors - Xing Ren ; KD Validated
human,mouse	WB,IHC	Bone Res; PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function; Authors - Yan Shao