PDRG1 Polyklonaler Antikörper

PDRG1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16968-1-AP

Synonyme

C20orf126, p53 and DNA damage regulated 1, PDRG, PDRG1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16968-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16968-1-AP (PDRG1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16968-1-AP (PDRG1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 16968-1-AP

  Immunofluorescent analysis of HepG2 cells, using PDRG1 antibody 16968-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

16968-1-AP bindet in WB, IHC, IF, ELISA PDRG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDRG1 fusion protein Ag9109
• Vollständiger Name: p53 and DNA damage regulated 1
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC009334
• Gene symbol: PDRG1
• Gene ID (NCBI): 81572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDRG1 codes for a novel protein called p53 and DNA damage-regulated protein 1. Human PDRG1 is predominantly expressed in normal testis and its expression can be induced by UV irradiation and be repressed by p53 [PMID14562055]. Recently, PDRG1 was identified as a novel tumor marker for multiple malignancies that is selectively regulated by genotoxic stress. Its expression was upregulated in multiple malignancies including cancers of the colon, rectum, ovary, lung, stomach, breast and uterus when compared to normal tissues [PMID: 21193842], suggesting its being a novel tumor marker that could play a role in cancer development and/or progression.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
mouse	IHC	DNA Repair (Amst); The influence of retinoic acid-induced differentiation on the radiation response of male germline stem cells.; Authors - Yi Zheng
human	WB	Ann Transl Med; A pan-cancer analysis of the expression and prognostic significance of PDRG1; Authors - Zhen Liu
Artemia sinica	WB	Int J Mol Sci; The Potential Roles of the Apoptosis-Related Protein PDRG1 in Diapause Embryo Restarting of Artemia sinica.; Authors - Wan Zhang