PDLIM7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDLIM7 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (2)

Kat-Nr. : 10221-1-AP

Synonyme

LMP1, LIM mineralization protein, ENIGMA

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10221-1-AP

  WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

• 

  WB analysis of SKOV-3 using 10221-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10221-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10221-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10221-1-AP

  IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:20000) with MCF-7 cells lysate 1000 ug.

• 

  IP experiment of MCF-7 using 10221-1-AP

  IP result of anti-PDLIM7 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000ug.

• 

  IHC staining of human ovary tumor using 10221-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of SKOV-3 using 10221-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using PDLIM7 antibody (10221-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

10221-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PDLIM7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDLIM7 fusion protein Ag0264
• Vollständiger Name: PDZ and LIM domain 7 (enigma)
• Berechnetes Molekulargewicht: 55/60 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC001093
• Gene symbol: PDLIM7
• Gene ID (NCBI): 9260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

Publikationen

Species	Application	Title
mouse	WB	EMBO Rep; Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.; Authors - Alon Abend
human,mouse	WB,IHC,IP	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	IF	J Bone Miner Res; LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.; Authors - Hehai Pan
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
human	IHC	J Endod; Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.; Authors - Wang Xiaoying X
human	WB	J Orthop Res; LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.; Authors - Hui Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tongbin (Verified Customer) (08-25-2020)

This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.

• Applications: Western Blot
• Cell Tissue Type: Heart

FH

Joleen (Verified Customer) (06-10-2019)

The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM7 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10221-1-AP)