PDLIM5 Polyklonaler Antikörper

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• KD/KO Validated

PDLIM5 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10530-1-AP

Synonyme

ENH, ENH1, Enigma homolog, L9, LIM, PDLIM5, PDZ and LIM domain 5, PDZ and LIM domain protein 5

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 10530-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 antibody) at dilution of 1:250.

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  WB analysis of HeLa using 10530-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 10530-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10530-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10530-1-AP (PDLIM5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 10530-1-AP

  IP result of anti-PDLIM5 (IP:10530-1-AP, 4ug; Detection:10530-1-AP 1:300) with A549 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 10530-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10530-1-AP (PDLIM5 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HUVEC-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

10530-1-AP bindet in WB, IHC, IF, IP, ELISA PDLIM5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDLIM5 fusion protein Ag0808
• Vollständiger Name: PDZ and LIM domain 5
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa
• GenBank-Zugangsnummer: BC008741
• Gene symbol: PDLIM5
• Gene ID (NCBI): 10611
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM5 (PDZ and LIM domain 5), formerly known as enigma homolog (ENH), is a cytoplasmic protein containing PDZ domain and LIM domain at N terminus and C terminus, respectively. PDLIM5 gene generates several splicing variants, EZH1 to EZH4. The long isoform, ENH1, is widely expressed in various tissues including heart, brain, spleen, liver and kidney. In comparison, shorter isoforms that lack the LIM motifs are expressed in cardiac (ENH3) and skeletal muscle (ENH2, ENH3, and ENH4). ENH1 is essential for differentiation of striated muscles through interaction with other proteins. In addition, PDLIM5 is also involved in mood disorders, such as schizophrenia, bipolar disorder, and major depression. This antibody is specific to PDLIM5, and its specificity has been confirmed by siRNA test.

Publikationen

Species	Application	Title
mouse	WB	Cell Death Differ; MicroRNA-17-92 regulates myoblast proliferation and differentiation by targeting the ENH1/Id1 signaling axis.; Authors - H Qiu ; KD Validated
human	WB,IHC	Oncotarget; High expression of PDLIM5 facilitates cell tumorigenesis and migration by maintaining AMPK activation in prostate cancer.; Authors - Xi Liu ; KD Validated
human	WB	Drug Deliv; Ultrasound-sensitive siRNA-loaded nanobubbles fabrication and antagonism in drug resistance for NSCLC.; Authors - Chunhong Su
rat	WB	J Mol Cell Cardiol; Extracellular vesicular MicroRNA-27a* contributes to cardiac hypertrophy in chronic heart failure.; Authors - Changhai Tian ; KD Validated
human	WB	Chem Biol Drug Des; si-RNA mediated knockdown of PDLIM5 suppresses gastric cancer cell proliferation in vitro.; Authors - Yanliang Li ; KD Validated
mouse	IHC	Sci Rep; Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration; Authors - Kento Takaya