PDK3 Polyklonaler Antikörper

PDK3 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12215-1-AP

Synonyme

PDK3

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12215-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12215-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12215-1-AP

  IP result of anti-PDK3 (IP:12215-1-AP, 4ug; Detection:12215-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

• 

  IHC staining of human skeletal muscle using 12215-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

12215-1-AP bindet in WB, IP, IHC, ELISA PDK3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDK3 fusion protein Ag2879
• Vollständiger Name: pyruvate dehydrogenase kinase, isozyme 3
• Berechnetes Molekulargewicht: 406 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015948
• Gene symbol: PDK3
• Gene ID (NCBI): 5165
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).

Publikationen

Species	Application	Title
human	WB	Cancer Res; IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism.; Authors - Jose L Izquierdo-Garcia
human	IHC	Front Oncol; Overexpression of Pyruvate Dehydrogenase Kinase-3 Predicts Poor Prognosis in Urothelial Carcinoma; Authors - Yu-Hsuan Kuo