PDIA3 Monoklonaler Antikörper

PDIA3 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G4G7

Kat-Nr. : CL488-66423

Synonyme

58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER marker, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, Mitochondria Marker, Mitochondrial Marker, P58, PDIA3, PI PLC, Protein disulfide isomerase A3

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Galerie der Validierungsdaten

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  IF Staining of HEK-293 using CL488-66423

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66423 (PDIA3 antibody) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66423 bindet in IF PDIA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDIA3 fusion protein Ag8741
• Vollständiger Name: protein disulfide isomerase family A, member 3
• Berechnetes Molekulargewicht: 505 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa, 60 kDa
• GenBank-Zugangsnummer: BC014433
• Gene symbol: PDIA3
• Gene ID (NCBI): 2923
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA3, also named as P58, ER60, ERp57, ERp60, ERp61, GRP57, GRP58 and PI-PLC, is a member of the PDI family, participates in the oxidation, reduction, and isomerization of disulfide bonds for correct folding of secretory proteins before modification and transport in the endoplasmic reticulum. It is associated with apoptosis or inhibition of cancer cell growth. PDIA3 was once thought to be a phospholipase; however, it has been demonstrated that this protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and PDIA3 mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.