PDE8A Polyklonaler Antikörper

PDE8A Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13956-1-AP

Synonyme

HsT19550, PDE8A, phosphodiesterase 8A

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 13956-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13956-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13956-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 13956-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 13956-1-AP

  IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug.

• 

  IHC staining of human colon cancer using 13956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 13956-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Mausmilzgewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

13956-1-AP bindet in WB, IP, IF, IHC, ELISA PDE8A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE8A fusion protein Ag5069
• Vollständiger Name: phosphodiesterase 8A
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 93 kDa, 88 kDa
• GenBank-Zugangsnummer: BC060762
• Gene symbol: PDE8A
• Gene ID (NCBI): 5151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; Inhibition of prolyl hydroxylases increases hepatic insulin and decreases glucagon sensitivity by an HIF-2α-dependent mechanism.; Authors - Matthew Riopel
pig	WB,IF	Sci Rep; Mitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cells.; Authors - Amel Lounas
		Reprod Fertil Dev; Active 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells.; Authors - Annick Bergeron
human	IF	Sci Rep; Disruption of the pro-oncogenic c-RAF-PDE8A complex represents a differentiated approach to treating KRAS-c-RAF dependent PDAC; Authors - Sean F Cooke
mouse	WB,IF	Neurochem Res; Phosphodiesterase 8 (PDE8): Distribution and Cellular Expression and Association with Alzheimer's Disease; Authors - Nian-Zhuang Qiu