PDE6A Monoklonaler Antikörper

PDE6A Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3B4

Kat-Nr. : 67832-1-Ig

Synonyme

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

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Galerie der Validierungsdaten

• 

  WB analysis of mouse eye using 67832-1-Ig

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse retina using 67832-1-Ig

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat eye using 67832-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe, Maus-Retina-Gewebe, Ratten-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67832-1-Ig bindet in WB,ELISA PDE6A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDE6A fusion protein Ag16741
• Vollständiger Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
• Berechnetes Molekulargewicht: 860 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC035909
• Gene symbol: PDE6A
• Gene ID (NCBI): 5145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.